stereo investigator 8.0 software program Search Results


stearic acid  (Chem Impex International)
95
Chem Impex International stearic acid
Stearic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stearic acid/product/Chem Impex International
Average 95 stars, based on 1 article reviews
stearic acid - by Bioz Stars, 2026-06
95/100 stars
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stomics stereo seq chips  (Complete Genomics Inc)
97
Complete Genomics Inc stomics stereo seq chips
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Stomics Stereo Seq Chips, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stomics stereo seq chips/product/Complete Genomics Inc
Average 97 stars, based on 1 article reviews
stomics stereo seq chips - by Bioz Stars, 2026-06
97/100 stars
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discovery v8 microscope  (Carl Zeiss)
97
Carl Zeiss discovery v8 microscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Discovery V8 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/discovery v8 microscope/product/Carl Zeiss
Average 97 stars, based on 1 article reviews
discovery v8 microscope - by Bioz Stars, 2026-06
97/100 stars
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stereo zoom microscope  (Nikon)
97
Nikon stereo zoom microscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Stereo Zoom Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stereo zoom microscope/product/Nikon
Average 97 stars, based on 1 article reviews
stereo zoom microscope - by Bioz Stars, 2026-06
97/100 stars
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acs apo 40× 1 15 oil  (Danaher Inc)
96
Danaher Inc acs apo 40× 1 15 oil
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Acs Apo 40× 1 15 Oil, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acs apo 40× 1 15 oil/product/Danaher Inc
Average 96 stars, based on 1 article reviews
acs apo 40× 1 15 oil - by Bioz Stars, 2026-06
96/100 stars
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m205 fa stereomicroscope  (Leica Microsystems)
96
Leica Microsystems m205 fa stereomicroscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
M205 Fa Stereomicroscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m205 fa stereomicroscope/product/Leica Microsystems
Average 96 stars, based on 1 article reviews
m205 fa stereomicroscope - by Bioz Stars, 2026-06
96/100 stars
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stereomicroscope  (Carl Zeiss)
97
Carl Zeiss stereomicroscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stereomicroscope/product/Carl Zeiss
Average 97 stars, based on 1 article reviews
stereomicroscope - by Bioz Stars, 2026-06
97/100 stars
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stereomicroscope zeiss v20  (Carl Zeiss)
96
Carl Zeiss stereomicroscope zeiss v20
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Stereomicroscope Zeiss V20, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stereomicroscope zeiss v20/product/Carl Zeiss
Average 96 stars, based on 1 article reviews
stereomicroscope zeiss v20 - by Bioz Stars, 2026-06
96/100 stars
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m 80 stereo microscope  (Leica Microsystems)
95
Leica Microsystems m 80 stereo microscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
M 80 Stereo Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m 80 stereo microscope/product/Leica Microsystems
Average 95 stars, based on 1 article reviews
m 80 stereo microscope - by Bioz Stars, 2026-06
95/100 stars
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stereomicroscope zeiss dr-c  (Carl Zeiss)
90
Carl Zeiss stereomicroscope zeiss dr-c
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Stereomicroscope Zeiss Dr C, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stereomicroscope zeiss dr-c/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
stereomicroscope zeiss dr-c - by Bioz Stars, 2026-06
90/100 stars
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cold light source olympus szx16 stereomicroscope  (Olympus)
96
Olympus cold light source olympus szx16 stereomicroscope
a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with <t>BGI</t> <t>Stereo-seq</t> technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.
Cold Light Source Olympus Szx16 Stereomicroscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cold light source olympus szx16 stereomicroscope/product/Olympus
Average 96 stars, based on 1 article reviews
cold light source olympus szx16 stereomicroscope - by Bioz Stars, 2026-06
96/100 stars
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Image Search Results


a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with BGI Stereo-seq technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.

Journal: bioRxiv

Article Title: YAP disrupts bile acid homeostasis to drive cancer-associated cachexia

doi: 10.64898/2026.02.01.702698

Figure Lengend Snippet: a , Schematic of the spatial transcriptomics (SRT) workflow. Cryosections were obtained from the indicated anatomical region (highlighted in schematic). Adjacent sister sections were stained with H&E, and sections for SRT were processed with BGI Stereo-seq technology. b , Spatial feature plot showing spot-level transcriptomic clustering of WT and PY-TB zebrafish samples. Clusters were identified by Seurat using principal component analysis and graph-based clustering of transcriptomic neighbourhoods. c , Integrated UMAP of all spatial transcriptomic spots coloured by Seurat-defined cluster identity. Cluster annotation was guided by regionally enriched marker genes and tissue interpretation using Zebrahub. d , UMAP and spatial feature plots highlighting hepatocyte-enriched spots defined by expression of hepatocyte marker, fabp10a , above the 75th percentile. Cells are grouped by sample: WT hepatocytes (blue) and PY-TB hepatocytes (red). e , Volcano plot of differentially expressed genes (DEGs) between PY-TB hepatocytes and WT hepatocytes, both defined by fabp10a expression in the spatial data. f , Spatial projection of cholangiocyte marker, anxa4, expression, visualised over spatial coordinates of the tissue section. g-i , Gene set enrichment analysis (GSEA) plots of selected pathways enriched in PY-TB hepatocytes versus WT, derived from DEGs identified in the SRT dataset. j , H&E and immunofluorescence staining of liver sections of WT and PY-TB fish at 21 dpf. Nuclei are marked with DAPI (cyan), hepatocytes with GFP (green), and cholangiocytes with ANXA4 (magenta). White arrow represents GFP+/ANXA4+ bi-lineage cells. Scale bar, 100 μm.

Article Snippet: Frozen tissue blocks were stored at −80 °C and cryosectioned at 10 μm onto STOmics Stereo-seq chips (V1.1; BGI Research).

Techniques: Spatial Transcriptomics, Staining, Marker, Expressing, Derivative Assay, Immunofluorescence